Microbeam-integrated multiphoton imaging system

Microbeam-integrated multiphoton imaging system.

Multiphoton microscopy has been added to the array of imaging techniques at the endstation for the Microbeam II cell irradiator at Columbia University's Radiological Research Accelerator Facility (RARAF). This three-dimensional (3D), laser-scanning microscope functions through multiphoton excitation, providing an enhanced imaging routine during radiation experiments with tissuelike samples, suc...

Optical workstation with concurrent, independent multiphoton imaging and experimental laser microbeam capabilities.

Experimental laser microbeam techniques have become established tools for studying living specimens. A steerable, focused laser beam may be used for a variety of experimental manipulations such as laser microsurgery, optical trapping, localized photolysis of caged bioactive probes, and patterned photobleaching. Typically, purpose-designed experimental systems have been constructed for each of t...

Brain Imaging with Multiphoton Microscopy

Fiber-based microendoscopic multiphoton imaging

We developed a multiphoton microscope which integrates an all normal dispersion fiber laser, a double cladding photonic crystal fiber and a MEMS mirror scanner based hand-held probe. The fiber laser has a central wavelength of 1.06um,a repetition rate of 76MHz and maximum average output power of more than 1W. The MEMS mirror based probe is compact and Second harmonic generation and two photon e...

Multiphoton Microscopy for Ophthalmic Imaging

We review multiphoton microscopy (MPM) including two-photon autofluorescence (2PAF), second harmonic generation (SHG), third harmonic generation (THG), fluorescence lifetime (FLIM), and coherent anti-Stokes Raman Scattering (CARS) with relevance to clinical applications in ophthalmology. The different imaging modalities are discussed highlighting the particular strength that each has for functi...